Treatment of chronic liver disease using cell‑engineered constructs: morphofunctional characteristics

Objective: to study the effectiveness of correcting morphofunctional characteristics liver in an experimental model chronic disease (CLD), using implanted cell-engineered constructs (CECs). Materials and methods. Experiments were carried out on male Wistar rats (n = 80) aged 6–8 months with initial weight 230–250 g. CLD was modeled by inoculating 60% CCl4 oil solution for 42 days based a modified scheme. Microgel recombinant spidroin rS1/9 used as matrix CECs fabrication. Allogeneic cells (LCs) multipotent bone marrow-derived mesenchymal stem (BM-MSCs) from healthy donor cellular component CECs. The corrective effect assessed 60) two groups rats: Group 1 (control, n 20, mL saline injected into damaged parenchyma) 2 (experimental, 40, containing allogenic LCs BM-MSCs 5 : ratio volume parenchyma). For long-term monitoring CEC state, labeled additional inclusion Cytodex-3. regulatory regenerative processes evaluated biochemical, morphological morphometric techniques, well flow cytometry at 90 after implantation. Results. In control group, mortality rate 25%. There no death group found have biochemical parameters during follow-up, concomitant preservation structural homeostasis Conclusion. Implantation facilitates effective correction activating liver, which is due